7JJT

Ruminococcus bromii amylase Amy5 (RBR_07800)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	98.460, 98.460, 196.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.001 - 1.660
R-factor	0.1612
Rwork	0.160
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wza
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MoRDa
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.001	1.719
High resolution limit [Å]	1.660	1.660
Number of reflections	114177	11264
<I/σ(I)>	10.58
Completeness [%]	99.9
Redundancy	9.8
CC(1/2)	0.997	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.02M Zinc Chloride and 20% PEG 3350