7JJM

Crystal structure of Importin alpha 2 in complex with LSD1 NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9357
Spacegroup name	P 21 21 21
Unit cell lengths	78.610, 90.050, 99.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.038 - 2.060
R-factor	0.2218
Rwork	0.220
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.002
RMSD bond angle	0.502
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.040	2.060
High resolution limit [Å]	2.060	2.060
Rmerge	0.159	0.759
Number of reflections	43845	2917
<I/σ(I)>	4.3
Completeness [%]	98.7
Redundancy	4.8
CC(1/2)	0.984	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7