7JJE
Sarcin-ricin loop with guanosine dithiophosphate residue.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 |
Unit cell lengths | 29.581, 29.581, 76.653 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.600 - 1.250 |
R-factor | 0.1122 |
Rwork | 0.110 |
R-free | 0.14780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 3S7C |
RMSD bond length | 0.011 |
RMSD bond angle | 1.745 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.065 | 0.510 |
Rpim | 0.028 | 0.239 |
Number of reflections | 18152 | 1812 |
<I/σ(I)> | 40.06 | 2.76 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Crystallization set-ups were made by mixing 4 uL of 350 uM RNA solution [1 mM Na 2 EDTA (pH 8.0) and 10 mM Tris-HCl (pH 8.0)] with 2 uL of a crystallization buffer composed of 3.0 M ammonium sulfate, 10 mM magnesium chloride, 10 mM manganese chloride, and 50 mM potassium 3-(N-morpholino) propanesulfonic acid (MOPS), pH 7.0 at 18 C. |