7JHX

Crystal structure of hEPG5 LIR/GABARAPL1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000010
Spacegroup name	C 1 2 1
Unit cell lengths	122.954, 33.082, 78.858
Unit cell angles	90.00, 114.28, 90.00

Refinement procedure

Resolution	57.000 - 1.910
R-factor	0.1991
Rwork	0.197
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r2q
Data reduction software	DIALS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.000	57.000	1.960
High resolution limit [Å]	1.910	8.540	1.910
Rmerge	0.148	0.067	0.762
Rmeas	0.177	0.081	0.916
Rpim	0.097	0.045	0.504
Total number of observations	68435	843	2867
Number of reflections	21346	289	989
<I/σ(I)>	4.6	8.9	1.6
Completeness [%]	93.4	99	58.7
Redundancy	3.2	2.9	2.9
CC(1/2)	0.985	0.985	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.2 M ammonium sulfate, 0.1 MES buffer pH 5.5, 29% (w/v) PEG 4000