7JHK

Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 in unliganded form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	87
Detector technology	CCD
Collection date	2017-12-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	67.416, 80.070, 157.614
Unit cell angles	90.00, 98.82, 90.00

Refinement procedure

Resolution	47.185 - 2.344
R-factor	0.1811
Rwork	0.179
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jhi
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.390
High resolution limit [Å]	2.344	6.380	2.350
Rmerge	0.070	0.036	0.437
Rmeas	0.078	0.041	0.508
Rpim	0.035	0.018	0.256
Number of reflections	66967	3616	2565
<I/σ(I)>	7.4
Completeness [%]	95.7	99.2	74.2
Redundancy	4.6	4.9	3.6
CC(1/2)		0.998	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	24% PEG1500, 20% glycerol