7JH8
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J20 immobile Holliday junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.074, 68.074, 56.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.037 - 3.087 |
| R-factor | 0.2487 |
| Rwork | 0.246 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xna |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.663 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.087 | 8.400 | 3.100 |
| Rmerge | 0.068 | 0.032 | 0.373 |
| Rmeas | 0.073 | 0.035 | 0.403 |
| Rpim | 0.026 | 0.013 | 0.148 |
| Number of reflections | 4830 | 269 | 172 |
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 90.3 | 97.8 | 62.5 |
| Redundancy | 7.1 | 7.3 | 5.6 |
| CC(1/2) | 0.993 | 0.998 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.5 with 30 mM MgCl2, 1.0 mM spermine, and 1.3 M Li2SO4 was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |
