7JH0
Crystallographic structure of glyceraldehyde-3-phosphate dehydrogenase from Schistosoma mansoni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | P 61 |
Unit cell lengths | 80.123, 80.123, 417.015 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.760 - 2.510 |
R-factor | 0.2158 |
Rwork | 0.213 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k9d |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.760 | 2.570 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmeas | 0.172 | 2.654 |
Number of reflections | 51375 | 3515 |
<I/σ(I)> | 14.35 | 1.13 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 21.57 | |
CC(1/2) | 0.999 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.12 M Ethylene glycols (0.3 M diethyleneglycol, 0.3 M triethyleneglycol, 0.3 M tetraethyleneglycol, 0.3 M pentaethyleneglycol) , 0.1 M Buffer system 1 pH 6.5 (0,1 M MES/Imidazole) and 30% Mix of precipitants (20% V/V Glicerol e 10% w/V PEG 40,000) |