7JGU

Structure of FN3tt mut

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	106.000, 38.310, 28.180
Unit cell angles	90.00, 103.88, 90.00

Refinement procedure

Resolution	27.360 - 1.200
R-factor	0.1505
Rwork	0.149
R-free	0.18090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jgt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.360	27.360	1.230
High resolution limit [Å]	1.200	5.370	1.200
Rmerge	0.050	0.035	0.923
Rmeas	0.060	0.042	1.128
Total number of observations	108762
Number of reflections	34160	404	2526
<I/σ(I)>	12.39	27.18	2.55
Completeness [%]	99.0	96.4	99.3
Redundancy	3.184	3.208	2.921
CC(1/2)	0.998	0.996	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	25% PEG 3350, 0.2M sodium di-hydrogen phosphate