7JFX

Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J16 immobile Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92
Spacegroup name	P 32
Unit cell lengths	68.150, 68.150, 53.785
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.020 - 3.210
R-factor	0.2463
Rwork	0.242
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xna
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.020	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.087	0.046	0.236
Rmeas	0.092	0.048	0.258
Rpim	0.029	0.015	0.103
Number of reflections	3636	227	107
<I/σ(I)>	3.7
Completeness [%]	79.0	100	43.9
Redundancy	8.4	10.3	4.2
CC(1/2)	0.879	0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.5 mL of 0.05 M Cacodylate pH 6.5 with 2.0 mM spermine, 1.0 mM CoH18N6, and 80 mM CaCl2 was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock