7JFT
Self-assembly of a 3D DNA crystal lattice (4x6 junction version) containing the J2 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 |
Unit cell lengths | 69.111, 69.111, 56.404 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.556 - 3.158 |
R-factor | 0.2255 |
Rwork | 0.224 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xna |
RMSD bond length | 0.006 |
RMSD bond angle | 0.773 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.200 |
High resolution limit [Å] | 3.150 | 8.540 | 3.150 |
Rmerge | 0.117 | 0.097 | 0.321 |
Rmeas | 0.127 | 0.106 | 0.361 |
Rpim | 0.048 | 0.041 | 0.162 |
Number of reflections | 4138 | 256 | 98 |
<I/σ(I)> | 6 | ||
Completeness [%] | 80.1 | 100 | 41.5 |
Redundancy | 6.6 | 8.1 | 3.2 |
CC(1/2) | 0.991 | 0.965 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 6.5 with 2.0 mM spermine, 1.0 mM CoH18N6, and 80 mM CaCl2 was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |