7JFS

The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54187
Spacegroup name	P 61
Unit cell lengths	185.743, 185.743, 81.591
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.930 - 4.600
R-factor	0.2633
Rwork	0.261
R-free	0.30920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kdn
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.680
High resolution limit [Å]	4.600	4.600
Rmerge	0.183	0.757
Rpim	0.084	0.387
Number of reflections	9041	449
<I/σ(I)>	8.6	1.9
Completeness [%]	99.7	100
Redundancy	4.7	4.4
CC(1/2)	0.981	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	2% Tascimate, 0.1 M Bis-Tris, 15% PEG3350