7JFM

Crystal structure of mouse phosphorylated IRF-3 bound to CBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2016-09-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	118.801, 118.801, 72.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.440 - 2.230
R-factor	0.2324
Rwork	0.231
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jem
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.440	2.300
High resolution limit [Å]	2.230	2.230
Rmerge	0.089
Rpim	0.027
Number of reflections	28422	2601
<I/σ(I)>	12.6
Completeness [%]	99.9
Redundancy	12.1
CC(1/2)	0.990	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.2 M ammonium citrate tribasic at pH 7.0, 12% PEG 3350