7HOB
PanDDA analysis group deposition -- Crystal Structure of TRIM21, ground state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-04 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.92134 |
| Spacegroup name | I 4 |
| Unit cell lengths | 95.338, 95.338, 45.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.730 - 1.150 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.18510 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.981 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.720 | 33.720 | 1.170 |
| High resolution limit [Å] | 1.150 | 3.120 | 1.150 |
| Rmerge | 0.040 | 0.035 | 0.298 |
| Number of reflections | 59061 | 3764 | 410 |
| <I/σ(I)> | 33.1 | 88.3 | 2.1 |
| Completeness [%] | 85.1 | 100 | 11.4 |
| CC(1/2) | 1.000 | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 4 % PEG 400, 2 M AmmSO4, 0.1 M HEPES pH 8 |
