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7HM2

PanDDA analysis group deposition -- Crystal Structure of TRIM21 in complex with Z32400357

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04-1
Synchrotron siteDiamond
BeamlineI04-1
Temperature [K]100
Detector technologyPIXEL
Collection date2024-05-24
DetectorDECTRIS EIGER2 XE 9M
Wavelength(s)0.92124
Spacegroup nameI 4
Unit cell lengths95.294, 95.294, 45.864
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution67.380 - 1.150
R-factor0.1806
Rwork0.180
R-free0.20020
Structure solution methodFOURIER SYNTHESIS
RMSD bond length0.013
RMSD bond angle1.930
Data reduction softwareXDS
Data scaling softwareAimless (0.7.7)
Phasing softwareREFMAC
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]67.38067.3801.170
High resolution limit [Å]1.1506.3001.150
Rmerge0.0480.0680.561
Rmeas0.0510.0710.793
Rpim0.0140.0230.561
Total number of observations5872656004465
Number of reflections62218480455
<I/σ(I)>23.979.80.7
Completeness [%]85.399.912.8
Redundancy9.412.51
CC(1/2)0.9980.9600.508
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP82934 % PEG 400, 2 M AmmSO4, 0.1 M HEPES pH 8

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