7HM0
PanDDA analysis group deposition -- Crystal Structure of TRIM21 in complex with Z374427992
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-24 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | I 4 |
| Unit cell lengths | 95.505, 95.505, 45.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.530 - 1.430 |
| R-factor | 0.1891 |
| Rwork | 0.188 |
| R-free | 0.21410 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.540 | 67.540 | 1.450 |
| High resolution limit [Å] | 1.430 | 7.700 | 1.430 |
| Rmerge | 0.139 | 0.037 | 5.227 |
| Rmeas | 0.145 | 0.039 | 5.535 |
| Rpim | 0.040 | 0.012 | 1.808 |
| Total number of observations | 486037 | 3317 | 17805 |
| Number of reflections | 38348 | 275 | 1963 |
| <I/σ(I)> | 9.7 | 59.4 | 0.3 |
| Completeness [%] | 100.0 | 99.9 | 99.6 |
| Redundancy | 12.7 | 12.1 | 9.1 |
| CC(1/2) | 0.999 | 1.000 | 0.353 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 4 % PEG 400, 2 M AmmSO4, 0.1 M HEPES pH 8 |
