7GAP
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z728939702
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 61.215, 61.215, 35.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.650 - 1.680 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.24360 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.630 | 30.630 | 1.710 |
| High resolution limit [Å] | 1.680 | 9.050 | 1.680 |
| Rmerge | 0.153 | 0.118 | 5.552 |
| Rmeas | 0.159 | 0.123 | 5.761 |
| Rpim | 0.043 | 0.036 | 1.530 |
| Total number of observations | 207746 | 1198 | 10646 |
| Number of reflections | 15116 | 110 | 760 |
| <I/σ(I)> | 9.9 | 47.9 | 0.3 |
| Completeness [%] | 99.6 | 98.6 | 98.8 |
| Redundancy | 13.7 | 10.9 | 14 |
| CC(1/2) | 0.977 | 0.998 | 0.327 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
