7GAL
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z291279160
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.872, 60.872, 35.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 1.910 |
| R-factor | 0.1957 |
| Rwork | 0.193 |
| R-free | 0.25100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.424 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.050 | 43.050 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.120 | 1.900 |
| Rmerge | 0.118 | 0.099 | 3.840 |
| Rmeas | 0.123 | 0.104 | 3.985 |
| Rpim | 0.033 | 0.031 | 1.059 |
| Total number of observations | 141587 | 1145 | 9231 |
| Number of reflections | 10401 | 109 | 656 |
| <I/σ(I)> | 9.2 | 45.4 | 0.3 |
| Completeness [%] | 99.9 | 99.2 | 99.2 |
| Redundancy | 13.6 | 10.5 | 14.1 |
| CC(1/2) | 0.999 | 0.999 | 0.509 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
