7GAC
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z1456069604
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.616, 60.616, 35.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.900 - 1.910 |
| R-factor | 0.2089 |
| Rwork | 0.207 |
| R-free | 0.25980 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.880 | 42.880 | 1.950 |
| High resolution limit [Å] | 1.910 | 8.960 | 1.910 |
| Rmerge | 0.125 | 0.117 | 5.458 |
| Rmeas | 0.131 | 0.126 | 5.665 |
| Rpim | 0.036 | 0.044 | 1.506 |
| Total number of observations | 140194 | 1229 | 9706 |
| Number of reflections | 10254 | 117 | 684 |
| <I/σ(I)> | 9.8 | 52.1 | 0.3 |
| Completeness [%] | 99.5 | 99.2 | 100 |
| Redundancy | 13.7 | 10.5 | 14.2 |
| CC(1/2) | 0.953 | 0.746 | 0.256 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
