7GAB
PanDDA analysis group deposition -- Crystal Structure of MAP1LC3B in complex with Z1255402624
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.892, 60.892, 35.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.090 - 2.230 |
| R-factor | 0.1902 |
| Rwork | 0.187 |
| R-free | 0.27550 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8q53 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.625 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.050 | 43.050 | 2.310 |
| High resolution limit [Å] | 2.230 | 8.930 | 2.230 |
| Rmerge | 0.144 | 0.052 | 3.603 |
| Rmeas | 0.150 | 0.055 | 3.747 |
| Rpim | 0.041 | 0.017 | 1.022 |
| Total number of observations | 87203 | 1223 | 7854 |
| Number of reflections | 6512 | 116 | 584 |
| <I/σ(I)> | 10.3 | 44.6 | 0.5 |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 13.4 | 10.5 | 13.4 |
| CC(1/2) | 0.996 | 0.990 | 0.279 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 36% PEG 8000, 0.1M acetate pH 4.7 |
