7G9F
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102209-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.700, 70.700, 195.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.560 - 1.935 |
| R-factor | 0.2205 |
| Rwork | 0.218 |
| R-free | 0.26030 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.650 | 97.650 | 1.980 |
| High resolution limit [Å] | 1.940 | 8.870 | 1.940 |
| Rmerge | 0.420 | 0.068 | |
| Rmeas | 0.429 | 0.069 | 43.306 |
| Rpim | 0.083 | 0.015 | 8.138 |
| Total number of observations | 1007040 | 9640 | 72847 |
| Number of reflections | 38030 | ||
| <I/σ(I)> | 6.3 | 42.9 | 0.2 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 26.5 | 19.1 | 27.9 |
| CC(1/2) | 0.998 | 0.999 | 0.456 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
