7G9D
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102179-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.693, 70.693, 195.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.390 - 2.659 |
| R-factor | 0.1994 |
| Rwork | 0.195 |
| R-free | 0.28620 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.480 | 66.480 | 2.800 |
| High resolution limit [Å] | 2.660 | 8.410 | 2.660 |
| Rmerge | 0.901 | 0.092 | |
| Rmeas | 0.925 | 0.096 | 11.181 |
| Rpim | 0.210 | 0.024 | 2.495 |
| Total number of observations | 282710 | 8251 | 41777 |
| Number of reflections | 15102 | ||
| <I/σ(I)> | 6.5 | 17.2 | 1.2 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 18.7 | 14.2 | 19.5 |
| CC(1/2) | 0.988 | 0.997 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
