7G99
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with PCM-0102102-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.926, 70.926, 195.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.690 - 1.780 |
| R-factor | 0.2077 |
| Rwork | 0.206 |
| R-free | 0.23660 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.040 | 98.040 | 1.820 |
| High resolution limit [Å] | 1.780 | 8.910 | 1.780 |
| Rmerge | 0.756 | 0.062 | |
| Rmeas | 0.770 | 0.063 | 139.653 |
| Rpim | 0.148 | 0.014 | 26.748 |
| Total number of observations | 1299863 | 9563 | 75213 |
| Number of reflections | 48956 | ||
| <I/σ(I)> | 6.6 | 37.5 | 0.3 |
| Completeness [%] | 99.9 | 100 | 99.1 |
| Redundancy | 26.6 | 19 | 26.6 |
| CC(1/2) | 0.998 | 0.999 | 0.268 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
