7G98
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z4605084898
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.804, 70.804, 196.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.600 - 2.878 |
| R-factor | 0.2131 |
| Rwork | 0.209 |
| R-free | 0.29460 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 196.260 | 196.260 | 3.030 |
| High resolution limit [Å] | 2.880 | 9.100 | 2.880 |
| Rmerge | 2.857 | 0.096 | |
| Rmeas | 2.947 | 0.100 | 62.893 |
| Rpim | 0.708 | 0.027 | 15.647 |
| Total number of observations | 207204 | 5912 | 27864 |
| Number of reflections | 12087 | ||
| <I/σ(I)> | 5.4 | 15.7 | 0.7 |
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 17.1 | 12.5 | 16.3 |
| CC(1/2) | 0.767 | 0.995 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
