7G96
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1203730981
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.720, 70.720, 196.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.520 - 2.300 |
| R-factor | 0.2013 |
| Rwork | 0.198 |
| R-free | 0.26570 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.720 | 70.720 | 2.200 |
| High resolution limit [Å] | 2.140 | 9.570 | 2.140 |
| Rmerge | 0.507 | 0.092 | |
| Rmeas | 0.517 | 0.094 | 15.137 |
| Rpim | 0.101 | 0.022 | 2.882 |
| Total number of observations | 740463 | 7131 | 55883 |
| Number of reflections | 28461 | ||
| <I/σ(I)> | 5.4 | 20.4 | 0.3 |
| Completeness [%] | 100.0 | 99.6 | 99.9 |
| Redundancy | 26 | 17.2 | 27.3 |
| CC(1/2) | 0.996 | 0.997 | 0.387 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
