7G8Y
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1449748885
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | DIAMOND |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.029, 71.029, 196.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.110 - 1.750 |
R-factor | 0.1934 |
Rwork | 0.192 |
R-free | 0.22210 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.009 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.750 | 32.750 | 1.780 |
High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
Rmerge | 0.305 | 0.060 | 5.888 |
Rmeas | 0.311 | 0.062 | 5.998 |
Rpim | 0.060 | 0.014 | 1.135 |
Total number of observations | 1376604 | 8816 | 76444 |
Number of reflections | 51686 | ||
<I/σ(I)> | 7.4 | 40.6 | 0.5 |
Completeness [%] | 99.8 | 98.2 | 99.2 |
Redundancy | 26.6 | 19.2 | 27.3 |
CC(1/2) | 0.997 | 0.998 | 0.377 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |