7G8M
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1255459547
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.035, 71.035, 196.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.660 - 2.029 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.25980 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 32.680 | 32.680 | 2.080 |
| High resolution limit [Å] | 2.030 | 9.080 | 2.030 |
| Rmerge | 0.438 | 0.070 | |
| Rmeas | 0.446 | 0.071 | 11.137 |
| Rpim | 0.086 | 0.015 | 2.087 |
| Total number of observations | 883874 | 9011 | 66860 |
| Number of reflections | 33456 | ||
| <I/σ(I)> | 5.9 | 35.5 | 0.4 |
| Completeness [%] | 99.9 | 98.2 | 99.8 |
| Redundancy | 26.4 | 19.5 | 27.8 |
| CC(1/2) | 0.996 | 0.999 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
