7G8D
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z104474228
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.379, 71.379, 196.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.960 - 1.937 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.23590 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.100 | 67.100 | 2.040 |
| High resolution limit [Å] | 1.940 | 6.130 | 1.940 |
| Rmerge | 0.469 | 0.090 | 8.544 |
| Rmeas | 0.478 | 0.092 | 8.700 |
| Rpim | 0.092 | 0.019 | 1.634 |
| Total number of observations | 1033059 | 32205 | 155214 |
| Number of reflections | 38913 | ||
| <I/σ(I)> | 7.2 | 25.3 | 0.6 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 26.5 | 22.5 | 27.9 |
| CC(1/2) | 0.997 | 0.999 | 0.398 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
