7G8C
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1037511924
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | DIAMOND |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.758, 71.758, 196.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.420 - 2.176 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25520 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.420 | 67.420 | 2.290 |
| High resolution limit [Å] | 2.180 | 6.880 | 2.180 |
| Rmerge | 0.621 | 0.088 | 7.080 |
| Rmeas | 0.634 | 0.090 | 7.223 |
| Rpim | 0.128 | 0.020 | 1.424 |
| Total number of observations | 669384 | 20570 | 98964 |
| Number of reflections | 28021 | ||
| <I/σ(I)> | 6.3 | 22.5 | 0.8 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 23.9 | 19.5 | 25 |
| CC(1/2) | 0.994 | 0.999 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
