7G83
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z108545814
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92124 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.353, 71.353, 196.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.970 - 1.310 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.20540 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 8bnt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.100 | 67.100 | 1.330 |
| High resolution limit [Å] | 1.310 | 7.060 | 1.310 |
| Rmerge | 0.099 | 0.044 | 3.883 |
| Rmeas | 0.101 | 0.045 | 4.052 |
| Rpim | 0.020 | 0.010 | 1.128 |
| Total number of observations | 2492949 | 20646 | 30231 |
| Number of reflections | 106753 | ||
| <I/σ(I)> | 16.9 | 74.7 | 0.6 |
| Completeness [%] | 87.3 | 99.9 | 40.3 |
| Redundancy | 23.4 | 21.7 | 12.2 |
| CC(1/2) | 0.999 | 0.999 | 0.383 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |
