7G81
ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z104474512
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | DIAMOND |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92124 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.455, 71.455, 196.906 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.730 - 1.510 |
R-factor | 0.1936 |
Rwork | 0.193 |
R-free | 0.21600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 8bnt |
RMSD bond length | 0.011 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.4 (14-JUN-2023)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.730 | 35.730 | 1.540 |
High resolution limit [Å] | 1.510 | 8.270 | 1.510 |
Rmerge | 0.278 | 0.060 | 5.619 |
Rmeas | 0.284 | 0.061 | 5.775 |
Rpim | 0.056 | 0.013 | 1.315 |
Total number of observations | 2040680 | 12371 | 74168 |
Number of reflections | 81060 | ||
<I/σ(I)> | 9 | 52.3 | 0.5 |
Completeness [%] | 100.0 | 99.1 | 99.5 |
Redundancy | 25.2 | 20 | 19 |
CC(1/2) | 0.999 | 0.999 | 0.298 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris, 2.6M sodium formate |