7FZE

ammonium sulfate-based apo human FABP4, primitive orthorhombic form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-06-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.999900
Spacegroup name	P 21 21 21
Unit cell lengths	32.649, 53.627, 75.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.510 - 1.170
R-factor	0.1383
Rwork	0.137
R-free	0.17220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.022
RMSD bond angle	2.169
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.510	37.510	1.200
High resolution limit [Å]	1.170	5.250	1.170
Rmerge	0.034	0.034	0.113
Rmeas	0.037	0.037	0.127
Total number of observations	271106
Number of reflections	44522	594	2947
<I/σ(I)>	32.31	56.36	10.72
Completeness [%]	99.0	99	89.9
Redundancy	6.089	5.955	4.446
CC(1/2)	0.999	0.998	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368