7FZA

Crystal Structure of apo human FABP4, cubic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-09
Detector	PSI PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 4 3 2
Unit cell lengths	137.105, 137.105, 137.105
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.474 - 2.601
R-factor	0.2007
Rwork	0.198
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.008
RMSD bond angle	1.166
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_707)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.700	48.474	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.126	0.031	4.330
Rmeas	0.155	0.032	4.394
Total number of observations	471794
Number of reflections	14121	211	1020
<I/σ(I)>	22.88	82.44	1.16
Completeness [%]	99.8	97.2	100
Redundancy	32.78	22.768	34.925
CC(1/2)	0.999	0.998	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368