7FY1

Crystal Structure of apo human FABP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	107.980, 107.980, 76.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.760 - 2.010
R-factor	0.2223
Rwork	0.220
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760	46.760	2.060
High resolution limit [Å]	2.010	8.990	2.010
Rmerge	0.077	0.043	9.995
Rmeas	0.078	0.044	11.456
Total number of observations	651695
Number of reflections	18103	260	1270
<I/σ(I)>	23.84	62.6	0.3
Completeness [%]	99.7	98.5	97.7
Redundancy	35.999	26.927	13.258
CC(1/2)	1.000	1.000	0.138

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368