7FWT

Crystal Structure of apo mouse FABP4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.700010
Spacegroup name	P 21 21 21
Unit cell lengths	49.981, 78.029, 94.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.230 - 1.540
R-factor	0.1584
Rwork	0.156
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.016
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.230	47.230	1.580
High resolution limit [Å]	1.540	6.890	1.540
Rmerge	0.042	0.017	1.528
Rmeas	0.046	0.019	1.656
Total number of observations	368300
Number of reflections	55284	728	4035
<I/σ(I)>	17.75	69.16	1.15
Completeness [%]	99.6	99.3	99.1
Redundancy	6.66	5.787	6.765
CC(1/2)	1.000	1.000	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368