7FTF
Crystal Structure of apo human cyclic GMP-AMP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999980 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 48.367, 59.896, 125.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.129 - 1.508 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3139) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.730 | 45.129 | 1.550 |
High resolution limit [Å] | 1.510 | 6.740 | 1.510 |
Rmerge | 0.051 | 0.030 | 2.782 |
Rmeas | 0.055 | 0.032 | 3.010 |
Total number of observations | 423586 | ||
Number of reflections | 58354 | 768 | 4254 |
<I/σ(I)> | 14.87 | 48.04 | 0.61 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 7.259 | 6.557 | 6.937 |
CC(1/2) | 0.999 | 0.999 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of the Procomplex screen. Several conditions resulted in crystals. |