7FPG
PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09F06 from the F2X-Universal Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.557, 82.034, 93.225 |
Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
Resolution | 43.140 - 1.600 |
R-factor | 0.2261 |
Rwork | 0.225 |
R-free | 0.26540 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.140 | 43.140 | 43.140 |
High resolution limit [Å] | 1.600 | 4.780 | 1.600 |
Rmerge | 0.038 | 4.057 | |
Rmeas | 0.063 | 0.041 | 4.381 |
Total number of observations | 555789 | 20990 | 82370 |
Number of reflections | 79261 | 3154 | 11844 |
<I/σ(I)> | 11.86 | 45.94 | 0.37 |
Completeness [%] | 95.9 | 98.5 | 89 |
Redundancy | 7.01 | 6.66 | 6.95 |
CC(1/2) | 0.999 | 0.997 | 0.239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate |