7FNX
PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F08 from the F2X-Universal Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.517, 81.456, 93.356 |
Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
Resolution | 40.730 - 1.720 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.25910 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.730 | 40.730 | 40.730 |
High resolution limit [Å] | 1.720 | 5.110 | 1.710 |
Rmerge | 0.035 | 2.891 | |
Rmeas | 0.072 | 0.038 | 3.135 |
Total number of observations | 466448 | 17824 | 69489 |
Number of reflections | 67726 | 2641 | 10357 |
<I/σ(I)> | 12.33 | 47.26 | 0.47 |
Completeness [%] | 99.0 | 99.4 | 94.2 |
Redundancy | 6.89 | 6.75 | 6.71 |
CC(1/2) | 0.999 | 0.999 | 0.250 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate |