7FJR
Structure of a mutant of OspA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.070, 65.070, 309.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.690 - 2.600 |
| R-factor | 0.2638 |
| Rwork | 0.262 |
| R-free | 0.29650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.536 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.690 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.344 |
| Number of reflections | 12935 | 1237 |
| <I/σ(I)> | 49.3 | 9.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | Protein: 16.0 mg/mL, 35% Polyethylene glycol 400, 0.1 M Tris-HCl |
