7FIV

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CidA and CidBND1-ND2 from wPip(Tunis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 41
Unit cell lengths	181.577, 181.577, 54.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.610 - 2.590
R-factor	0.198
Rwork	0.196
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7esy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.590	7.050	2.600
Rmerge	0.190	0.089	1.312
Rmeas	0.199	0.093	1.414
Rpim	0.058	0.027	0.506
Number of reflections	56251	2978	2772
<I/σ(I)>	4.9
Completeness [%]	100.0	100	99.6
Redundancy	11.1	11.4	6.8
CC(1/2)		0.997	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289.15	0.1M BICINE pH 8.5, 15% w/v Polyethylene glycol 1500