7FIP
The native structure of beta-1,2-mannobiose phosphorylase from Thermoanaerobacter sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | BRUKER METALJET |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-10-28 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.34138 |
Spacegroup name | I 4 |
Unit cell lengths | 206.317, 206.317, 78.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.380 - 2.390 |
R-factor | 0.1888 |
Rwork | 0.187 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b0r |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.400 | 2.430 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.130 | 0.414 |
Number of reflections | 127183 | 3164 |
<I/σ(I)> | 7.2 | 2.3 |
Completeness [%] | 99.9 | |
Redundancy | 4.3 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 10 mM ZnCl2, 26% PEG3350, 180 mM Tris-HCl pH 7.0 |