7FI2

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473-H168A from Stenotrophomonas maltophilia (strain K279a) at pH-5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-12-23
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	75.564, 75.564, 112.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.309 - 2.600
Rwork	0.187
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhu
RMSD bond length	0.008
RMSD bond angle	1.568
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.440	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.114	0.331
Number of reflections	11515	1013
<I/σ(I)>	16.59
Completeness [%]	96.4	78.3
Redundancy	10.07	4.25
CC(1/2)	0.996	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298.15	0.2M LiSo4 Monohydrate, 0.1M Sodium acetate trihdyrate pH-5.0 25% (w/v) PEG 3350