7FHY
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-07-21 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.652, 57.943, 67.569 |
| Unit cell angles | 71.14, 89.71, 73.79 |
Refinement procedure
| Resolution | 29.052 - 2.200 |
| Rwork | 0.221 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fhu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.568 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.090 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.142 | 0.445 |
| Number of reflections | 30028 | 3689 |
| <I/σ(I)> | 9.33 | 2.94 |
| Completeness [%] | 98.7 | 97 |
| Redundancy | 5.24 | 3.24 |
| CC(1/2) | 0.991 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298.15 | 0.2M LiSO4 Monohydrdate, 100mM Sodium Cacodylate, pH-7.0, 30% (w/v) PEG 8000 |
