7FHR
Crystal Structure of a Rieske Oxygenase from Cupriavidus metallidurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.7377 |
| Spacegroup name | P 63 |
| Unit cell lengths | 103.416, 103.416, 77.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.710 - 1.840 |
| R-factor | 0.1668 |
| Rwork | 0.164 |
| R-free | 0.21180 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.719 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.590 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 40987 | 2488 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | glycine, lysine, glutamate, PEG 4000 |
