7FHP

Crystal structure of an orphan heme uptake protein (MhuP) of ABC transporter from Mycobacterium tuberculosis (Form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-01-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	202.380, 39.670, 92.970
Unit cell angles	90.00, 113.38, 90.00

Refinement procedure

Resolution	53.250 - 2.010
R-factor	0.1737
Rwork	0.171
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fhm
RMSD bond length	0.014
RMSD bond angle	1.785
Data reduction software	MOSFLM (7.3.0)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.250	53.250	2.060
High resolution limit [Å]	2.010	8.980	2.010
Rmerge	0.117	0.082	0.290
Rmeas	0.135	0.098	0.342
Rpim	0.067	0.052	0.177
Number of reflections	45608	584	3241
<I/σ(I)>	9.8
Completeness [%]	99.0	99.2	96.1
Redundancy	3.9	3.6	3.3
CC(1/2)	0.985	0.980	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	0.2 M lithium sulfate monohydrate, 0.1 M sodium acetate trihydrate pH 4.6, 30% (w/v) PEG 8000