7FHE

Structure of prenyltransferase mutant Q295F from Streptomyces sp. (strain CL190)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	43.831, 75.970, 79.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.450 - 2.500
R-factor	0.2092
Rwork	0.205
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.450	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.154	1.015
Rmeas	0.160	1.056
Rpim	0.044	0.289
Total number of observations	122458
Number of reflections	9690	1073
<I/σ(I)>	11.4
Completeness [%]	99.9	100
Redundancy	12.6	13.1
CC(1/2)	0.998	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v PEG 20,000