7FHB

Structure of prenyltransferase from Streptomyces sp. (strain CL190) with bound GPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	44.462, 78.360, 84.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 1.900
R-factor	0.1786
Rwork	0.176
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb6
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.121	0.073	0.700
Rmeas	0.132	0.079	0.780
Rpim	0.051	0.030	0.335
Total number of observations	156859
Number of reflections	23861	1344	1077
<I/σ(I)>	9.2
Completeness [%]	98.8	100	89.2
Redundancy	6.6	6.6	5
CC(1/2)		0.993	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v PEG 20,000