7FH5

Structure of AdaV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	140.597, 140.597, 48.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.000
R-factor	0.1873
Rwork	0.187
R-free	0.20110
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.082
Number of reflections	37196	2719
<I/σ(I)>	23.9
Completeness [%]	99.9
Redundancy	19.8
CC(1/2)		0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.085 M HEPES sodium pH7.5, 1.7% v/v Polyethylene glycol 400 1.7 M Ammonium sulfate 15% v/v Glycerol