7FGU

H/D exchanged Hen egg-white lysozyme denatured in basic conditions and refolded in solution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2017-08-23
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	79.256, 79.256, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.440 - 2.300
Rwork	0.219
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k8g
RMSD bond length	0.006
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	2.420	2.070
High resolution limit [Å]	2.000	2.300	2.000
Rmerge	0.032	0.285	0.098
Number of reflections	8548	600	769
<I/σ(I)>	64
Completeness [%]	99.2
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	NACL, SODIUM ACETATE-D3, ACETIC ACID-D4, 100% D2O, PH 4.6
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	NACL, SODIUM ACETATE-D3, ACETIC ACID-D4, 100% D2O, PH 4.6