7FGK
The Fab antibody single structure against tau protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-05-17 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.140, 70.780, 41.550 |
| Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
| Resolution | 27.820 - 2.300 |
| R-factor | 0.20441 |
| Rwork | 0.199 |
| R-free | 0.29907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hk3 5eor |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.651 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.820 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.458 |
| Number of reflections | 22009 | 22009 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 20% PEG 3350, 15% Glycerol, 0.1M Ammonium iodide, 10mM Tris-HCl |
