7FGB

A naturally-occurring neuraminidase-inhibitors-resistant NA from asiatic toad influenza B-like virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	120.446, 143.150, 120.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.640 - 2.000
R-factor	0.2315
Rwork	0.229
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9s
RMSD bond length	0.008
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rpim	5.600	31.700
Number of reflections	70237	6890
<I/σ(I)>	12.6
Completeness [%]	98.7
Redundancy	11.6
CC(1/2)	0.992	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate pH 5.0, 26% v/v polyethylene 200.